Coding
Part:BBa_K1777005
Designed by: Xuhang Li Group: iGEM15_Fudan (2015-09-13)
flexible linker with SV40 NLS
((natural flexible linker)-(SV40 NLS)-(artificial GS linker))2 | |
---|---|
Function | Protein linker |
Use in | Mammalian cells |
RFC standard | RFC 10 |
Backbone | pSB1C3 |
Submitted by | [http://2015.igem.org/Team:Fudan Fudan 2015] |
This is an improved flexible linker offering around 50 Angstrom separation for fusion domains.Specially, this linker was designed with a SV40 NLS, and its codon is optimized for mammalian.So it can be well used to link different protein domains for fusion proteins locating in cell nucleus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/human
//direction/forward
//proteindomain/linker
//direction/forward
//proteindomain/linker
chassis | mammalian |
device_type | linker |
direction | forward |
signalling_molecule | SV40 NLS |